Identification and Detection of Streptococcus MUTANs in Plaque Samples
نویسندگان
چکیده
Streptococcus mutans have been proposed as the main causative agent of dental cavities. Dental caries is among the most common diseases in the world, this study was done to determine whether PCR analysis is useful to identify and even classify oral and other streptococci. 20 dental plaque samples were collected from teeth, 10 normal sample and 10 from persons with oral problems. The samples were diluted and plated in Mitis-Salivary media, Bacterial DNA was extracted from all specimens, then analysed by PCR with 16s universal primer and S. mutans serotype-specific primer sets. Then it was sequenced, The strains reported in this paper were identified as S. mutans on the basis of the fact that the sequence generated was Streptococcus mutans.
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